Analysis Note

ControlHuman tooth sections

Application

Research Sub CategoryOsteobiology

Research CategoryStem Cell Research

This Anti-DMP-1 (C-terminus) Antibody, clone 8G10.3 is validated for use in IH, DB for the detection of DMP-1 (C-terminus).

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in rat mandibular sections. (Baba, O., et al. (2004). Matrix Biology. 23:371–379.)

Dot Blot Analysis: A representative lot was used by an independent laboratory in DB. (Baba, O., et al. (2004). Matrix Biology. 23:371–379.)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dentin matrix protein 1, also known as DMP-1, is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in DMP-1 are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia.

Immunogen

Epitope: C-terminus

57 kDa fragments isolated from rat bone

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Immunohistochemistry in human tooth sections.

Immunohistochemistry Analysis: 1:50-1:250 dilution of this antibody detected DMP-1 in human tooth sections.

Specificity

This antibody detects the C-Terminus of DMP-1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

53 kDa calculated